Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis #H295R_CellsJanuary 12, 2019
We showed that human adrenocortical H295R cells grown under starvation conditions acquire a hyperandrogenic steroid profile with changes in steroid metabolizing enzymes HSD3B2 and CYP17A1 essential for androgen production.
In this study we investigated the gene expression profiles and specific signaling pathways in androgen producing H295R cells to obtain further insight into androgen regulation in humans.
We used gene expression profiling, bioinformatics analysis and signal transduction analysis to identify the genes involved in the regulatory network of androgen biosynthesis.
We found that starvation in human adrenal H295R cells induced androgen production ( Fig.
Figure 3 Effect of serum starvation on the cell cycle in H295R cells.
Calculations for an effect of RARB and ATRA on HSD3B2 activity revealed a significant increase (data not shown) confirming that RARB has a role in regulating adrenal androgen production.
We have previously shown that serum starvation enhances the androgen production in H295R cells by increasing the CYP17A1-17,20-lyase activity and repressing HSD3B2 expression and activity 12 .
RARB has been shown to interact with Nur77 46 , a well-known transcription factor for regulating HSD3B2 47 , therefore, we studied the co-operation between RARB and Nur77 in regulating HSD3B2.
For cell cycle analysis H295R cells were cultured under normal growth and starvation conditions.
& Miller, W. L. Transcription of the human genes for cytochrome P450scc and P450c17 is regulated differently in human adrenal NCI-H295 cells than in mouse adrenal Y1 cells .